Determination of angiotensin I-converting enzyme activity in equine blood: Lack of agreement between methods of analysis

De Mello Costa, M. F. and Carmon, A. K. and Alves, M. F. M. and Ryan, T. M. and Davies, H. M. and Anderson, G. A. and Slocombe, R. F. (2011) Determination of angiotensin I-converting enzyme activity in equine blood: Lack of agreement between methods of analysis. Journal of Veterinary Science, 12 (1). pp. 21-25. ISSN 1976-555X

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Abstract or Summary

Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore, in this study, we evaluated the level of agreement between three different assays using equine plasma obtained from 29 horses. Two spectrophotometric assays using Furylacryloyl- phenylalanyl-glycyl-glycine as substrate and one fluorimetric assay utilizing o-aminobenzoic acid-FRK-(Dnp)P-OH were employed. The results revealed that the measurements from the different assays were not in agreement, indicating that the methods should not be used interchangeably for measurement of equine ACE activity. Rather, a single method of analysis should be adopted to achieve comparable results and critical appraisal of the literature is needed when attempting to compare results obtained from different assays.

Item Type:Journal article
Keywords that describe the item:angiotensin I-converting enzyme, assay agreement, biochemistry, equine, methodology
Subjects:Q Science > QD Chemistry
Q Science > QP Physiology
Divisions:Schools > Centre for Science and Primary Industries
ID Code:1506
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Deposited On:09 Jan 2012 00:03
Last Modified:20 Aug 2014 01:50

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