Xie, Congmin Z. and Healy, Terry and Robinson, Peter and Stewart, Kevin (2008) Determination of EDTA in dairy wastewater and adjacent surface water. In: Proceedings of World Academy of Science, Engineering and Technology. World Academy of Science, Engineering and Technology, pp. 50-54.
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Available under License Creative Commons Attribution.
Official URL: http://www.waset.org/pwaset/v34/v34-9.pdf
Abstract—An HPLC-UV analytical method was developed to determine ethylenediaminetetraacetic acid (EDTA) in dairy wastewater and surface water. The optimizing separation was achieved by reversed–phase ion-pair liquid chromatography on a C18 column using methanol as mobile phase solvent, tetrabutylammonium bromide as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate of 0.9 mL min-1 with a UV detector at 265 nm. No interference of Ca, Mg or NO3- was detected. Method performance was evaluated in terms of linearity, repeatability and reproducibility. The method detection limit was 5 μg L-1. The contents of EDTA in dairy effluents were 72-261 μg L-1 at a large dairy site. A change of EDTA concentration was observed downstream of the dairy effluent discharge, but this was well under the predicted no effect concentration for aquatic ecosystem.
|Item Type:||Book Section|
|Additional Information:||Conference held 29-31 October, 2008, in Venice, Italy|
|Keywords:||Dairy wastewater, EDTA, HPLC, surface water.|
|Subjects:||Q Science > Q Science (General)|
|Divisions:||Schools > School of Science and Primary Industries|
|Deposited On:||17 Jun 2009 03:34|
|Last Modified:||09 Apr 2010 02:33|
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