Determination of EDTA in dairy wastewater and adjacent surface water

Xie, Congmin Z. and Healy, Terry and Robinson, Peter and Stewart, Kevin (2008) Determination of EDTA in dairy wastewater and adjacent surface water. In: Proceedings of World Academy of Science, Engineering and Technology. World Academy of Science, Engineering and Technology, pp. 50-54.

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Abstract or Summary

Abstract—An HPLC-UV analytical method was developed to determine ethylenediaminetetraacetic acid (EDTA) in dairy wastewater and surface water. The optimizing separation was achieved by reversed–phase ion-pair liquid chromatography on a C18 column using methanol as mobile phase solvent, tetrabutylammonium bromide as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate of 0.9 mL min-1 with a UV detector at 265 nm. No interference of Ca, Mg or NO3- was detected. Method performance was evaluated in terms of linearity, repeatability and reproducibility. The method detection limit was 5 μg L-1. The contents of EDTA in dairy effluents were 72-261 μg L-1 at a large dairy site. A change of EDTA concentration was observed downstream of the dairy effluent discharge, but this was well under the predicted no effect concentration for aquatic ecosystem.

Item Type:Book Section
Additional Information:Conference held 29-31 October, 2008, in Venice, Italy
Keywords that describe the item:Dairy wastewater, EDTA, HPLC, surface water.
Subjects:Q Science > Q Science (General)
Divisions:Schools > School of Science and Primary Industries
ID Code:235
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Deposited On:17 Jun 2009 03:34
Last Modified:09 Apr 2010 02:33

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