Validation of high throughput comet assay to study gut health effects of prebiotics

McElroy, Amber and Moorhead, S. M. (2013) Validation of high throughput comet assay to study gut health effects of prebiotics. In: New Zealand Microbiological Society Conference, 26-28 November 2013, Hamilton, New Zealand.

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Official URL: http://www.nzms2013.org.nz/

Abstract or Summary

The single cell gel or comet assay is a method used to determine the genotoxicity of substances. It is however both time and material intensive. In order to overcome these limitations a high throughput method using 96-well multi chamber plates was examined as a method to analyse the effect of a prebiotic (inulin) on detoxification of 2-amino-1- methyl-6-phenylimidazo (4, 5-b) pyridine (PhIP) [1], a carcinogenic heterocyclic amine formed during cooking of meat, using an in vitro model of colonic fermentation with human faecal microbiota

Item Type:Conference or Workshop Item (Poster)
Keywords that describe the item:genotoxicity
Subjects:Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions:Schools > Centre for Science and Primary Industries
ID Code:3076
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Deposited On:04 Mar 2014 21:24
Last Modified:23 Apr 2014 01:54

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