Targeting and molecular imaging of HepG2 cells using surface-functionalized gold nanoparticles

Rathinaraj, Pierson and Kang, InnKyu (2015) Targeting and molecular imaging of HepG2 cells using surface-functionalized gold nanoparticles. Targeting and molecular imaging of HepG2 cells using surface-functionalized gold nanoparticles, 17 (7). pp. 311-318. ISSN DOI10.1007/s11051-015-3118-y

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Abstract or Summary

Mercaptosuccinic acid (M)-conjugated gold nanoparticles (GM) were prepared and characterized by transmission electron microscope and dynamic light scattering. M was used to improve the monodispersity and non-specific intracellular uptake of nanoparticles. Lactobionic acid (L) was subsequently conjugated to the GM to target preferentially HepG2 cells (liver cancer cells) that express asialoglycoprotein receptors (ASGPR) on their membrane surfaces and facilitate the transit of nanoparticles across the cell membrane. The mean size of lactobionic acid-conjugated gold nanoparticle (GL) was approximately 10 ± 0.2 nm. Finally, the Atto 680 dye (A6) was coupled to the nanoparticles to visualize their internalization into HepG2 cells. The interaction of surface-modified gold nanoparticles with HepG2 cells was studied after culturing cells in media containing the GM or L-conjugated GM (GL).

Item Type:Journal article
Keywords that describe the item:HepG2 cells, molecular imaging, surface functionalized gold nanoparticles
Subjects:Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions:Corporate > Research Office
ID Code:5691
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Deposited On:17 Jan 2018 20:32
Last Modified:17 Jan 2018 20:32

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