Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells

Rathinaraj, Pierson and Gunaseelan, Srithar (2017) Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells. Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells, 12 (5). ISSN CC BY 4.0

[img]
Preview
PDF (Article) - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

22Mb

Official URL: https://www.researchgate.net/publication/316660564...

Abstract or Summary

Ultraviolet-B radiation (285–320 nm) elicits a number of cellular signaling elements. We investigated the preventive effect of linalool, a natural monoterpene, against UVB-induced oxidative imbalance, activation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) signaling in HDFa cells. We observed that linalool treatment (30 μM) prevented acute UVB-irradiation (20 mJ/cm 2) mediated loss of activities of antioxidant enzymes in HDFa cells. The comet assay results illustrate that linalool significantly prevents UVB-mediated 8-deoxy guanosine formation (oxidative DNA damage) rather than UVB-induced cyclobutane pyrimidine (CPD) formation. This might be due to its ability to prevent UVB-induced ROS formation and to restore the oxidative imbalance of cells. This has been reflected in UVB-induced overexpression of MAPK and NF-κB signaling. We observed that linalool inhibited UVB-induced phosphorylation of ERK1, JNK and p38 proteins of MAPK family. Linalool inhibited UVB-induced activation of NF-κB/p65 by activating IκBa. We further observed that UVB-induced expression of TNF-α, IL6, IL-10, MMP-2 and MMP-9 was modulated by linalool treatment in HDFa cells. Thus, linalool protects the human skin cells from the oxidative damages of UVB radiation and modulates MAPK and NF-κB signaling in HDFa cells. The present findings substantiate that linalool may act as a photoprotective agent against UVB-induced skin damages.

Item Type:Journal article
Keywords that describe the item:Linalool, oxidative stress, human skin cells
Subjects:Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions:Corporate > Research Office
ID Code:5721
Deposited By:
Deposited On:17 Jan 2018 22:29
Last Modified:17 Jan 2018 22:29

Repository Staff Only: item control page