Enhanced Intracellular Uptake of CdTe Quantum Dots by Conjugation of Oligopeptides

Moon, Jeong Choi and Rathinaraj, Pierson (2013) Enhanced Intracellular Uptake of CdTe Quantum Dots by Conjugation of Oligopeptides. Journal of Nanomaterials, 2013 (291020). pp. 1-8.

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Official URL: https://www.researchgate.net/publication/258391852...

Abstract or Summary

Arg-Gly-Asp-Ser (RGDS), a typical membrane-permeable carrier peptide, was conjugated with mercaptoisobutyric acid-immobilized CdTe quantum dot (CTNPs) to enhance the intracellular uptake of quantum dots. Mean size of mercaptoisobutyric acidimmobilized quantum dots (37 nm) as determined by dynamic light scattering was increased up to 54 nm after RGDS immobilization. It was found, from in vitro cell culture experiment, that fibroblast (NIH 3T3) cells were well proliferated in the presence of RGDS-conjugated quantum dots (RCTNPs), and the intracellular uptake of CTNPs and RCTNPs was studied by means of ICP and fluorescence microscopy. As a result, the RCTNPs specifically bound to the membrane of NIH 3T3 cells and almost saturated after 6 hours incubation. The amount of RCTNPs uptaken by the cells was higher than that of CTNPs, demonstrating the enhancing effect of RGDS peptide conjugation on the intracellular uptake of quantum dots (QDs).

Item Type:Journal article
Keywords that describe the item:Enhanced Intracellular
Subjects:Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions:Corporate > Research Office
ID Code:5753
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Deposited On:19 Jan 2018 02:47
Last Modified:19 Jan 2018 02:53

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