Polo is an equestrian team sport, consisting of Open and Women’s only handicapping systems. Equine activities may differ in distribution and their affect upon match outcome in Women’s Polo compared to Open Polo, potentially impacting equine preparation and management. We aimed to quantify spatiotemporal differences between Open and Women’s Polo when matched for handicap and assess their interaction with chukka and match outcomes. Distance, speed and high-intensity activity data were collected via player-worn global positioning system (GPS) units during 16-goal Open and Women’s Polo tournaments. Notational analysis quantified chukka duration and chukka and game outcomes. Spatiotemporal metric differences between Open and Women’s Polo were small to large (ES: 0.54–1.81). In Open Polo, players covered moderately more distance in games won (mean: 429.0 m; 95% CI: 238.9 m to 619.0 m), with small to large increases in high-intensity activities also performed. Whereas in Women’s Polo, moderately higher maximum speeds were attained in games won (17.13 km.h−1; 11.86 km.h−1 to 22.40 km.h−1) and a small increase in accelerations performed (5.1; 0.2 to 10.0). Open and Women’s Polo, when matched for handicap, present with small to large spatiotemporal differences that are likely of practical significance, and may influence game outcome differently between codes.